[Oral Presentation]Effects of microbes and coal-derived humic acid substance on the growth of packchoi and soil amendment for coal mine subsided area

Effects of microbes and coal-derived humic acid substance on the growth of packchoi and soil amendment for coal mine subsided area

ID：66 Submission ID：226 View Protection：ATTENDEE Updated Time：2024-05-27 15:23:20 Hits：423 Oral Presentation

Start Time：2024-05-31 14:00 （Asia/Shanghai）

Duration：15min

Session：[S6] Clean Processing, Conversion and Utilization of Energy Resources » [S6-2] Afternoon of May 31st

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Abstract

Coal mining poses a potential threat to soil ecological environments, drawing increasing global attention to mitigate such disturbances. One approach involves utilizing coal-derived humic acid (CHA) and microbial inoculants to improve soil properties and foster crop growth. However, the effects of combining CHA and microbial inoculants on both crop growth and soil characteristics have not been well studied. In this study, we conducted a pot experiment using pakchoi (Brassica chinensis L.) and soil samples obtained from a coal mine subsidence area in Jiawang, Xuzhou, China. CHA was added in conjugation with microbial inoculants possessing lignite degradation abilities. The study aimed to evaluate the response of soil physical and chemical properties, enzyme activity, microbial community structure, and pakchoi growth to different gradients of CHA. In addition, detailed correlations among these factors to assess the role of CHA in amending soil and promoting plant growth were also conducted. Our findings revealed that CHA led to an increase in small macroaggregate distribution in soil accompanied by a decrease in soil pH and electrical conductivity. Furthermore, CHA enhanced nitrogen and phosphorus utilization by pakchoi and improved soil enzyme (urease, sucrase, and catalase) activity. Although different CHA dosages had minimal influence on indigenous bacterial community abundance, they exhibited significant relationships with fungal abundance. Notably, the dominant phylum and genus of fungi were Ascomycota and Gibberella, respectively. The introduction of CHA significantly promoted pakchoi root development and leaf growth, contributing to the accumulation of soluble sugar and protein in pakchoi. Soil parameters such as nitrogen, sucrose activity, phosphorus, and pH represented significant correlations with bacteria and fungi. Abundant soil bacteria were found to be beneficial for pakchoi growth, while the main fungal phylum Mortierellomycota contributed to soil nutrient transformation, facilitating leaf emergence and dry matter accumulation. This study provides valuable insights into the application of CHA for soil amendment in coal mine subsidence areas and illustrates its potential roles in enhancing soil quality and promoting plant growth.